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lentiviral psmal celltag v1 plasmid library  (Addgene inc)


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    Addgene inc lentiviral psmal celltag v1 plasmid library
    Lentiviral Psmal Celltag V1 Plasmid Library, supplied by Addgene inc, used in various techniques. Bioz Stars score: 93/100, based on 12 PubMed citations. ZERO BIAS - scores, article reviews, protocol conditions and more
    https://www.bioz.com/product/lentiviral+library+plasmid+pool/Morris+CellTag+Barcode+Library+(Pooled+Libraries+%23115643%2C+%23115643-LVC%2C+%23115644%2C+%23115644-LVC%2C+%23115645%2C+%23115645-LVC)/pmc12830798-247-1-5
    Average 93 stars, based on 12 article reviews
    lentiviral psmal celltag v1 plasmid library - by Bioz Stars, 2026-10
    93/100 stars

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    Plasmid Preparation:

    Article Title: Generating long deletions across the genome with pooled paired prime editing screens
    Article Snippet: The final library was extracted with the QIAGEN Plasmid Plus Midi Kit. .. To produce lentivirus in HEK293FT cells, 32.4 μg of the lentiviral library plasmid pool was mixed with 32.4 μg of psPax2 (Addgene 12260) and 7.2 μg of pMD2.G (Addgene 12259) in Opti-MEM (Gibco) together with 72 μl of PLUS reagent (Invitrogen). .. After 5 min of incubation at room temperature, 216 μl of LTX (Invitrogen) was added, and the mix was further incubated for 30 min. Then, 3 ml were dropped onto 10-cm dishes with 80% confluent cells in DMEM medium.



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